Fig 1: STIM2ß knockout inhibited NFAT4 and MEF2c. (A) Comparative NFAT4 and MEF2c mRNA expression by RT-PCR between wild-type and STIM2ß knockout cells. (B,C) GAPDH-normalized expression levels of NFAT4 (B) and MEF2c (C) of wild-type cells (Black circle) and STIM2ß knockout cells (White circle) during myogenesis. (D) Immunoblot analysis of wild-type cells and STIM2ß knockout cells to measure the protein expression of MEF2C during myogenesis. (E) Actin-normalized expression levels of MEF2C in wild-type cells (Black circle) and STIM2ß knockout cells (White circle). (F) The fraction of MEF2c positive nuclei of wild-type cell (Black circle) and STIM2ß over-expressed cells (White rectangular). (n > 60 cells for each group) (G) The fraction of MEF2c positive nuclei of wild-type cell (Black circle), STIM2ß knockout cells (White circle) and transiently STIM2ß expressed STIM2ß knockout cells (Black rectangular). (n > 60 cells for each group). Error bars show means ± SEM. The results are representative of at least three independent experiments.